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A reignited debate over the cell(s) of origin for glioblastoma and its clinical implications
Xiaolin Fan, Yanzhen Xiong, Yuan Wang
《医学前沿(英文)》 2019年 第13卷 第5期 页码 531-539 doi: 10.1007/s11684-019-0700-1
关键词: glioblastoma cell(s) of origin neural stem cells oligodendrocyte precursor cells subventricular zone early diagnosis
《医学前沿(英文)》 doi: 10.1007/s11684-023-1017-7
关键词: single-cell RNA-seq glioma radial glia primitive oligodendrocyte precursor cell immune escape
Early T-cell precursor leukemia: a subtype of high risk childhood acute lymphoblastic leukemia
null
《医学前沿(英文)》 2012年 第6卷 第4期 页码 416-420 doi: 10.1007/s11684-012-0224-4
Acute lymphoblastic leukemia includes T-cell acute lymphoblastic leukemia (T-ALL) and B-cell acute lymphoblastic leukemia (B-ALL). In children, T-ALL usually has a worse prognosis than B-ALL, although childhood T-ALL prognoses have improved remarkably. The varying outcomes among T-ALL cases suggest that an unrecognized biological heterogeneity may contribute to chemo-resistance. Deep exploration of T-lymphocyte development in recent years has found a subgroup of patients with a phenotype that resembles early T-cell precursor, which confers a much poorer prognosis than any other form of T-ALL. This novel subtype of T-ALL was called early T-cell precursor acute lymphoblastic leukemia (ETP-ALL). Flow cytometry data from T-ALL patients enrolled in Shanghai Children’s Medical Center between July 2002 and October 2010 were assessed according to Dr. Campana’s protocol. Among total 89 T-ALL cases, 74 cases had enough immunophenotype data available to differentiate between ETP (CD1a-, CD8-, CD5dim, at least one marker of stem cell or myeloid lineage) and non-ETP. From these 74 subjects, 12 ETP-ALL cases (16.2%) were identified. The event-free survival (EFS) rate at 66.8 months was 11.1%±10.1% for ETP-ALL and 57.6%±5.6% for non-ETP-ALL (P=0.003). The overall survival rates were 13.3%±11.0% for ETP-ALL and 64.7%±6.3% for non-ETP-ALL (P=0.002). Our findings demonstrate that early T-cell precursor leukemia is a very high-risk subtype of acute lymphoblastic leukemia with poor prognosis.
关键词: acute lymphoblastic leukemia early T precursor prognosis
Ripen NSENGA MD, Longxian CHENG PhD, Mei’an HE PhD, Tangchun WU PhD,
《医学前沿(英文)》 2009年 第3卷 第4期 页码 437-442 doi: 10.1007/s11684-009-0074-x
关键词: natriuretic peptide precursor A coronary heart disease gene polymorphism allelic discrimination polymorphism single nucleotide
Carbon-coated lithium titanate: effect of carbon precursor addition processes on the electrochemical
Shilei Ding, Zelong Jiang, Jing Gu, Hongliang Zhang, Jiajia Cai, Dongdong Wang
《化学科学与工程前沿(英文)》 2021年 第15卷 第1期 页码 148-155 doi: 10.1007/s11705-020-2022-x
关键词: lithium titanate carbon-coated carbon precursor synthetic process
W. Widiyastuti, Adhi Setiawan, Sugeng Winardi, Tantular Nurtono, Heru Setyawan
《化学科学与工程前沿(英文)》 2014年 第8卷 第1期 页码 104-113 doi: 10.1007/s11705-014-1406-1
关键词: droplet hydroxyapatite particle CFD tubular furnace spray pyrolysis
Emerging molecular subtypes and therapeutic targets in B-cell precursor acute lymphoblastic leukemia
Jianfeng Li, Yuting Dai, Liang Wu, Ming Zhang, Wen Ouyang, Jinyan Huang, Saijuan Chen
《医学前沿(英文)》 2021年 第15卷 第3期 页码 347-371 doi: 10.1007/s11684-020-0821-6
关键词: BCP-ALL subtypes translocation aneuploidy sequence mutations
Hydrothermal synthesis of crystalline α-/β-MnO2 nanorods via γ-MnOOH nanorod precursors
ZHANG Weixin, REN Xiangbin, YANG Zeheng, WANG Hua, WANG Qiang, HUANG Fei
《化学科学与工程前沿(英文)》 2007年 第1卷 第4期 页码 365-371 doi: 10.1007/s11705-007-0066-9
关键词: obtained -MnOOH precursor Besides high-resolution transmission precursor
Porcine pluripotent stem cells: progress, challenges and prospects
Jianyong HAN, Yi-Liang MIAO, Jinlian HUA, Yan LI, Xue ZHANG, Jilong ZHOU, Na LI, Ying ZHANG, Jinying ZHANG, Zhonghua LIU
《农业科学与工程前沿(英文)》 2019年 第6卷 第1期 页码 8-27 doi: 10.15302/J-FASE-2018233
Pluripotent stem cells (PSCs) are characterized by their capacity for high self-renewal and multiple differentiation potential and include embryonic stem cells, embryonic germ cells and induced PSCs. PSCs provide a very suitable model for the studies of human diseases, drugs screening, regenerative medicine and developmental biology research. Pigs are considered as an ideal model for preclinical development of human xenotransplantation, therapeutic approaches and regenerative medicine because of their size and physiological similarity to humans. However, lack of knowledge about the derivation, characterization and pluripotency mechanisms of porcine PSCs hinders progress in these biotechnologies. In this review, we discuss the latest progress on porcine PSCs generation, evaluation criteria for pluripotency, the scientific and technical questions arising from these studies. We also introduce our perspectives on porcine PSC research, in the hope of providing new ideas for generating naive porcine PSCs and animal breeding.
关键词: embryonic germ cells embryonic stem cells induced pluripotent stem cells pigs pluripotent stem cells
Determination of telomerase activity in stem cells and non-stem cells of breast cancer
LI Zhi, HE Yanli, ZHANG Jiahua, ZHANG Jinghui, HUANG Tao
《医学前沿(英文)》 2007年 第1卷 第3期 页码 294-298 doi: 10.1007/s11684-007-0056-9
Innate and adaptive T cells in influenza disease
null
《医学前沿(英文)》 2018年 第12卷 第1期 页码 34-47 doi: 10.1007/s11684-017-0606-8
Influenza is a major global health problem, causing infections of the respiratory tract, often leading to acute pneumonia, life-threatening complications and even deaths. Over the last seven decades, vaccination strategies have been utilized to protect people from complications of influenza, especially groups at high risk of severe disease. While current vaccination regimens elicit strain-specific antibody responses, they fail to generate cross-protection against seasonal, pandemic and avian viruses. Moreover, vaccines designed to generate influenza-specific T-cell responses are yet to be optimized. During natural infection, viral replication is initially controlled by innate immunity before adaptive immune responses (T cells and antibody-producing B cells) achieve viral clearance and host recovery. Adaptive T and B cells maintain immunological memory and provide protection against subsequent infections with related influenza viruses. Recent studies also shed light on the role of innate T-cells (MAIT cells, gd T cells, and NKT cells) in controlling influenza and linking innate and adaptive immune mechanisms, thus making them attractive targets for vaccination strategies. We summarize the current knowledge on influenza-specific innate MAIT and gd T cells as well as adaptive CD8+ and CD4+ T cells, and discuss how these responses can be harnessed by novel vaccine strategies to elicit cross-protective immunity against different influenza strains and subtypes.
关键词: influenza innate T cells CD4+ and CD8+ T cells vaccination
Reprogramming of the pig primordial germ cells into pluripotent stem cells: a brief review
Qijing LEI, Qin PAN, Shuai YU, Na LI, Shulin CHEN, Kuldip SIDHU, Jinlian HUA
《农业科学与工程前沿(英文)》 2019年 第6卷 第1期 页码 28-32 doi: 10.15302/J-FASE-2018222
Primordial germ cells (PGCs) are regarded as unipotent cells that can produce only either spermatogonia or oocytes. However, PGCs can be converted into the pluripotent state by first dedifferentiation to embryonic germ cells and then by reprogramming to induce them to become pluripotent stem cells (iPSCs). These two stages can be completely implemented with mouse cells. However, authentic porcine iPSCs have not been established. Here, we discuss recent advances in the stem cell field for obtaining iPSCs from PGCs. This knowledge will provide some clues which will contribute to the regulation of reprogramming to pluripotency in farm species.
关键词: pig pluripotent stem cells primordial germ cells reprogramming
4-1BBL expressed by eukaryotic cells activates immune cells and suppresses the progression of murine
Hui QIU, Hui ZHANG, Zuohua FENG
《医学前沿(英文)》 2009年 第3卷 第1期 页码 20-25 doi: 10.1007/s11684-009-0006-9
关键词: 4-1BB ligand tumor immunotherapy tumor microenvironment
The past, present and future of bovine pluripotent stem cells: a brief overview
Xiuchun TIAN
《农业科学与工程前沿(英文)》 2019年 第6卷 第1期 页码 3-7 doi: 10.15302/J-FASE-2018247
Although the pursuit of bovine embryonic stem cells started more than 26 years ago for the purpose of gene-targeting, true pluripotent stem cells in this economically important species are still elusive. With the rapid advances in genome-editing and cloning using homologously recombined somatic cells, the need for pluripotent stem cells for precise genetic modification in any species became questionable. With the pig being the better model for human regenerative biology, the identification of the commonalities and uniqueness of the pluripotency circuitry across mammalian species may be the main objective for studying pluripotent stem cells in the bovine.
Comparison of human nasal epithelial cells grown as explant outgrowth cultures or dissociated tissue
null
《医学前沿(英文)》 2013年 第7卷 第4期 页码 486-491 doi: 10.1007/s11684-013-0287-x
The purpose of this study was to compare cell growth characteristics, ciliated cell differentiation, and function of human nasal epithelial cells established as explant outgrowth cultures or dissociated tissue cultures. Human nasal mucosa of the uncinate process was obtained by endoscopy and epithelial cell cultures were established by explant outgrowth or dissociated tissue culture methods. Epithelial cell growth characteristics were observed by inverted phase contrast microscopy. Ciliated cell differentiation was detected by β-tubulin IV and ZO-1 immunocytochemistry. Basal and ATP-stimulated ciliary beat frequency (CBF) was measured using a high-speed digital microscopic imaging system. Both the explant and dissociated tissue cultures established as monolayers with tight junctions and differentiated cell composition, with both types of cultures comprising ciliated and non-ciliated epithelial cells. Fibroblasts were also frequently found in explant cultures but rarely seen in dissociated tissue cultures. In both culture systems, the highest ciliated cell density appeared at 7th–10th culture day and declined with time, with the lifespan of ciliated cells ranging from 14 to 21 days. Overall, 10% of the cells in explant cultures and 20% of the cells in the dissociated tissue cultures were ciliated. These two cultures demonstrated similar ciliary beat frequency values at baseline (7.78±1.99 Hz and 7.91±2.52 Hz, respectively) and reacted equivalently following stimulation with 100 μM ATP. The results of this study indicate that both the explant outgrowth and dissociated tissue culture techniques are suitable for growing well-differentiated nasal ciliated and non-ciliated cells, which have growth characteristics and ciliary activity similar to those of nasal epithelial cells in vivo.
关键词: ciliated cells ciliary beat frequency dissociated tissue culture explant culture nasal epithelial cells
标题 作者 时间 类型 操作
A reignited debate over the cell(s) of origin for glioblastoma and its clinical implications
Xiaolin Fan, Yanzhen Xiong, Yuan Wang
期刊论文
Distinct immune escape and microenvironment between RG-like and pri-OPC-like glioma revealed by single-cell RNA-seq analysis
期刊论文
Early T-cell precursor leukemia: a subtype of high risk childhood acute lymphoblastic leukemia
null
期刊论文
The role of natriuretic peptide precursor A gene polymorphism in the development of coronary heart disease
Ripen NSENGA MD, Longxian CHENG PhD, Mei’an HE PhD, Tangchun WU PhD,
期刊论文
Carbon-coated lithium titanate: effect of carbon precursor addition processes on the electrochemical
Shilei Ding, Zelong Jiang, Jing Gu, Hongliang Zhang, Jiajia Cai, Dongdong Wang
期刊论文
Particle formation of hydroxyapatite precursor containing two components in a spray pyrolysis process
W. Widiyastuti, Adhi Setiawan, Sugeng Winardi, Tantular Nurtono, Heru Setyawan
期刊论文
Emerging molecular subtypes and therapeutic targets in B-cell precursor acute lymphoblastic leukemia
Jianfeng Li, Yuting Dai, Liang Wu, Ming Zhang, Wen Ouyang, Jinyan Huang, Saijuan Chen
期刊论文
Hydrothermal synthesis of crystalline α-/β-MnO2 nanorods via γ-MnOOH nanorod precursors
ZHANG Weixin, REN Xiangbin, YANG Zeheng, WANG Hua, WANG Qiang, HUANG Fei
期刊论文
Porcine pluripotent stem cells: progress, challenges and prospects
Jianyong HAN, Yi-Liang MIAO, Jinlian HUA, Yan LI, Xue ZHANG, Jilong ZHOU, Na LI, Ying ZHANG, Jinying ZHANG, Zhonghua LIU
期刊论文
Determination of telomerase activity in stem cells and non-stem cells of breast cancer
LI Zhi, HE Yanli, ZHANG Jiahua, ZHANG Jinghui, HUANG Tao
期刊论文
Reprogramming of the pig primordial germ cells into pluripotent stem cells: a brief review
Qijing LEI, Qin PAN, Shuai YU, Na LI, Shulin CHEN, Kuldip SIDHU, Jinlian HUA
期刊论文
4-1BBL expressed by eukaryotic cells activates immune cells and suppresses the progression of murine
Hui QIU, Hui ZHANG, Zuohua FENG
期刊论文